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Files in this Data Supplement:
Fig. S1. Expression of galectin 1 mRNA in developing and germ cell-depleted testes. (A) Whole mount ISH of a P4 mouse seminiferous tubule with a galectin 1 probe (purple). Note the segmental organization of the galectin 1 high, medium and low regions. (B,C) ISH of P14 (B) and P21 (C) testis sections with a galectin 1 probe. A higher magnification view of the region outlined by the rectangle in B is shown in B¢; those outlined by the left and right squares in C are shown in C¢ and C¢¢, respectively. At both stages, seminiferous tubule sections represent variable levels of galectin 1 mRNA. In the galectin 1-high segments, germ cells may be also positive for the galectin 1 signal, as suggested by Timmons et al. (Timmons et al., 2002). At both stages, seminiferous tubule segments with high galectin 1 contain young spermatocytes as early leptotene or zygotene (black arrows) and late pachytene or diplotene spermatocytes (black arrowheads). At P21, meiotic figures (indicated by dots) are also observed (C¢). By contrast, earlier pachytene spermatocytes (green arrows) and round spermatids (in P21, green arrowheads) are observed in segments with a low level of galectin 1. These combinations are compatible with those observed in adults (Timmons et al., 2002; Russell et al., 1990). (D-F) ISH of adult W/Wv testis (D), cryptorchid testis (E) and normal wild-type testis (F) sections probed for galectin 1. Note the variable levels of galectin 1 mRNA among seminiferous tubule segments in germ cell-depleted situations (E,F), similar to the normal situation (F). For B-F, specimens were counterstained with Nuclear Fast Red. Scale bars: 100 mm.
Russell, L., Ettlin, R., Sinha Hikim, A. and Clegg, E. (1990). Histological and Histopathological Evaluation of the Testis. Clearwater, Fl: Cache River Press.
Timmons, P. M., Rigby, P. W. and Poirier, F. (2002). The murine seminiferous epithelial cycle is pre-figured in the Sertoli cells of the embryonic testis. Development 129, 635-647.
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