|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Notch activation in the mouse cardiac cell lineage. (A-D)
Notch1 expression revealed by whole-mount in situ hybridization at
E10.5. In wild-type embryos (A,B), it is difficult to detect Notch1 expression
in the heart at the whole-mount level, whereas in NICD1-activated hearts
(C,D), Notch1 is strongly induced in whole heart which shows an
abnormal morphology. The heart regions are magnified (B,D). (E-L)
Immunohistochemical analysis of cleaved-Notch1 (NICD1 Ab) at E10.5. NICD1
signals are only observed in the nuclei of endocardial cells in the wild-type
heart (E-H), whereas the signals are observed in both endocardial and
myocardial cells in NICD1-activated hearts (I-L). The images of other sections
were generated to indicate that only endocardial cells are NICD1-positive in
the AV cushion of the wild-type embryonic heart (H). Arrows indicate
endocardial cells. Arrowheads indicate myocardial cells. Abbreviations: LA,
left atrium; RA, right atrium; LV, left ventricle; RV, right ventricle. Scale
bars: 200 µm in E,I; 50 µm in F-H,J-L.
|
