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Fig. 2. In situ hybridization for XNr1 in untreated and
concanamycin-treated Xenopus embryos. Treatment of
Xenopus embryos with the H+-V-ATPase inhibitor
concanamycin causes significant levels of heterotaxia that can be seen in the
situs of organs at stage 45 (Fig.
1L), but disruptions of laterality can be detected much earlier by
the expression patterns of normally left-sided markers. (A-D) Sectioned
embryos processed for in situ hybridization with an XNr1 antisense
probe. In wild-type embryos, XNr1 is restricted to the left
(A); however, in inhibitor-treated embryos, its expression is
randomized (B-D). Green arrows indicate normal position of staining;
red arrows indicate ectopic expression domain; white arrows indicate lack of
expression in normal region. Dorsal is upwards in all panels; left and right
sides correspond to those of the reader.
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