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Fig. 1. T-DNA insertion alleles of LMI1. (A) Map of the LMI1
locus. Exons are shown as rectangles, introns as black lines. The homeodomain
is indicated in red, the adjacent leucine zipper motif in blue. The four SALK
insertion lines analyzed are indicated as triangles. The distance between the
lines connecting each insertion with the sequence indicates the size of the
deletion in each insertion. The lmi1 allele designation and SALK
number are indicated. (B) Semi-quantitative RT-PCR analysis of
LMI1 expression in four-day-old seedlings of the insertion lines
compared with wild type (Col). PCR was performed with primers flanking the
insertions (LMI1; primers L5' and L3' in A) or upstream
of the lmi1-1 insertion (LMI1up; primers Lu5' and
Lu3' in A). The eukaryotic translation initiation factor 4A
(EIF4) was amplified as an internal control.
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