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Fig. 6. Altered histone methylation and acetylation at the SOC1 locus
in msi1-tap1 seedlings. (A) Scheme of the transcribed
region of the SOC1 locus and the fragments used for chromatin
immunoprecipitation (ChIP). The gray and black boxes symbolize exons and
introns, respectively, and the dark gray boxes, the untranslated exons.
(B) Expression of the phosphofructokinase (PFK) gene
At4g04040 is not changed in msi1-tap1 plants. RNA was
extracted from total 15-day-old seedlings grown on plates containing MS medium
in SD. (C) Quantification of PCR products after ChIP with
anti-dimethyl-histone H3K4 and anti-acetyl-histone H3K9 antiserum. Values were
normalized to PFK and are shown as relative enrichments in samples
from msi1-tap1 versus wild-type seedlings. The gray and hatched bars
represent the results of two independent ChIP experiments. Chromatin was
extracted from 15-day-old seedlings grown in SD harvested at 1 hour before the
end of the light period.
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