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Figure 1


Fig. 1. Nestin-Cre mediated conditional disruption of aPKC{lambda} gene. (A) Activity of ß-galactosidase in Nestin-Cre;Rosa26R embryo on a sagittal section at E15.5 is detected by X-gal staining to show tissue specificity of Cre-recombinase activity. Cre activity is relatively low in the caudal neocortical region (arrowhead indicates caudal and rostral boundary). (B) Proteins extracted from the telencephalon at E13.5 and E15.5 were analyzed by western blotting using an aPKC{lambda}-specific antibody (upper panel) or antibody recognizing all three aPKC members: aPKC{lambda}, aPKC{zeta} and PKM{zeta} (middle panel). Positions of aPKC{lambda} (70 kDa), aPKC{zeta} (70 kDa) and PKM{zeta} (55 kDa) are indicated by arrowheads. Signals detected with ß-actin antibody served as internal controls for equal protein loading (lower panel). Te, telencephalon; Di, diencephalon; Me, mesencephalon; Mt, metencephalon; My, myelencephalon; Sc, spinal cord; nc, neocortical region; ge, ganglionic eminence. Scale bar: 1 mm.





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