Na,K-ATPase 2 and Ncx4a regulate zebrafish left-right patterning

DEV02851 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. Cilia in KV are motile. This footage shows a dorsal view inside KV in a control embryo at the 10-somite stage. Multiple motile cilia can be seen in this focal plane.

• Movie 2 -

  Movie 2. Motile cilia are absent in KV of an α2 morphant.

• Movie 3 -

  Movie 3. Counterclockwise KV fluid flow in CTL-MO injected embryos. Fluorescent beads injected into KV show a net counterclockwise movement.

• Movie 4 -

  Movie 4. Fluid flow inside KV is disrupted in α2 morphants. Directional movement of fluorescent beads is absent in KV of α2-MO-injected embryos.

• Movie 5 -

  Movie 5. Cilia in KV of KN62-treated α2 morphants are motile.

• Movie 6 -

  Movie 6. Counterclockwise KV fluid flow is restored in α2 morphants by KN62 treatment.