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Fig. 3. Spatial and temporal expression of AtSWC6. (A)
Northern blot analysis of AtSWC6 expression in different
Arabidopsis tissues. RNA was extracted from 20-day-old Col grown in
long-day conditions for whole plants (Wh), root (Rt), shoot apex (SA) and
rosette leaves (RL). RNA for old leaves (OL), stem (St) and inflorescence with
flowers (Inf) was extracted from 40-day-old plants. (B) Temporal
expression of AtSWC6 assessed by RT-PCR. RNA was extracted from 5-,
8-, 11- and 14-day-old Col grown in long-day conditions. All the samples were
harvested 4 hours after light on. (C) Diurnal rhythm of AtSWC6
expression assessed by RT-PCR. RNA was extracted from 10-day-old Col grown in
long-day conditions. Northern blot analysis with the same RNA showed diurnal
rhythm in SUF3 expression. (D) The effect of photoperiod and
vernalization on AtSWC6 expression. FRI with (+) and without
(-) vernalization, and Col grown under long-day (LD) and short-day (SD)
conditions were used for northern blot and RT-PCR analyses. For RT-PCR
analysis, TUB expression served as an internal control. (E)
Spatial expression patterns of AtSWC6 were examined by GUS staining
in the AtSWC6p-AtSWC6:GUS transgenic plant. Histochemical GUS
staining was performed in whole (a) 3-, (b) 5- and (c) 10-day-old plants, in
(d) inflorescence and flowers, (e) an axillary bud, (f,g) developing seeds and
(g) siliques of different stages.
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