DEV02863 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Additional amnion-specific genes. A. gambiae (A-C) and D. melanogaster (D-F) embryos were hybridized with the indicated RNA probes. (A,B,D,E) rho1 expression patterns. Staining is detected in the ventral midline (E) and dorsal ectoderm (D) of D. melanogaster embryos. Comparable patterns in the ventral midline (B) and amnion (A, white arrow) are detected in A. gambiae embryos. (C,F) ind expression patterns. Staining is restricted to the lateral neurogenic ectoderm in D. melanogaster (F), but is seen in both the neurogenic ectoderm and anterior amnion (white arrow) in A. gambiae (C).

• Supplemental Figure S2 -

  Fig. S2. Mosquito zen is an activator. Wild-type (A,B) and transgenic (C,D) Drosophila embryos were co-hybridized with an Anopheles zen RNA probe (green channel) together with a D. melanogaster race RNA probe (red channel). The transgenic D. melanogaster embryos (C,D) express the mosquito zen gene under the control of a UAS cassette, driven by Kr-zen/GAL4, which mediates expression in the amnioserosa and in a transverse abdominal stripe. The wild-type embryo (A,B) does not stain green owing to the absence of mosquito zen expression. The transgenic embryo (C,D) exhibits an abnormal stripe of mosquito zen expression (green channel in C). The visualization of the single red channel (D. melanogaster race) in D shows sporadic ectopic activation of the endogenous race gene in the abdomen (arrows, D).

• Supplemental Figure S3 -

  Fig. S3. Anopheles gambiae sog locus