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Fig. 7. CS-GAGs are necessary for neuronal differentiation of precursors in
vivo. (A) Photomicrographs of immunohistochemical stainings of
E14.5 mouse forebrain cryosections 1 day after ventricular injection of ACSF
control (upper panels) or ChABC (lower panels). Cell nuclei were
counterstained with bisbenzimide and are shown in blue (DAPI). The prominent
presence of the 473HD epitope on cells with radial morphology in the germinal
layers of control sections was lost after ChABC injection, as expected.
Concomitantly, a substantial reduction in young postmitotic
ßIII-tubulin-positive neurons in the germinal layers of the cortex and
ganglionic eminence was observed. Note, however, that we observed an
accumulation of neurons in the cortical plate (lower right-hand panel).
(B) The changes were quantified by counting immunopositive cells 2
hours after plating of single-cell suspensions obtained from embryos injected
with ACSF or ChABC. Note that ChABC treatment caused a clear reduction in the
number of ßIII-tubulin-positive cells. We also recorded a small increase
in the number of GFAP-positive cells. **, P<0.01;
***, P<0.001. Scale bar: 100 µm.
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