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Figure 6


Fig. 6. Stat6 signalling is intact in KIM-2 cells. KIM-2 cells respond to IL-4 (A) and IL-13 (B) in a dose-dependent manner, confirmed by immunoblotting using phosphorylated (p)Stat6 and Stat6 antibodies. (C) KIM-2 medium was changed and then cells were harvested at various time points after treatment with or without 40 ng/ml of recombinant murine IL-13 (rmIL-13). Quantitative real-time-PCR was performed for Il4r{alpha}1 and Gata3; * denotes Student's t-test P value of <0.02. (D) Immunoblots for the same time course were performed for pStat6, Stat6 and {alpha}-tubulin. (E-G) KIM-2 cells were treated with brefeldin A (10 µg/ml) for 4 hours, fixed and stained with anti-IL-4 (E) or anti-IL-13 (F) antibodies or no antibody (G).





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