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Fig. 7. Th-2 cytokines are upregulated and secreted by differentiating KIM-2
cells. (A) Quantitative real-time-PCR was performed using specific
primers for the Th1 cytokines Il12a, Tnfa and Ifng (right)
and the Th2 cytokines Il4, Il13 and Il5 (left) on KIM-2 cells across
a differentiation time course (DD, days of differentiation). Error bar
represents s.d. of three samples and is representative of at least two
experiments. (B) Cytokine protein array demonstrates Th2 cytokine
production by differentiated KIM-2 cells at day 8 (right) compared to day 0
(left). Th2 cytokines are indicated in red. Green arrows indicate elevated
levels of IL-4 secretion at day 8 of differentiation. Pos, positive control;
Neg, negative control; GM-CSF, granulocyte-macrophage colony-stimulating
factor; GCSF, granulocyte-colony stimulating factor.
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