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Fig. 2. ERK and ELK1 are phosphorylated during mouse preimplantation
development. (A) Phosphorylated ERK (p-ERK, upper) and
phosphorylated ELK1 (p-ElK-1, lower) were detected using anti-phospho-ERK
antibody and anti-phospho-ELK1 antibody, respectively. Embryos (from left to
right) at the two-cell, four-cell, eight-cell, morula or blastocyst stages
were fixed and stained. Fluorescence was viewed with a confocal microscope.
(B) Phosphorylation of ERK or ELK1 with or without U0126. Four-cell
(top) and two-cell (bottom) stage embryos were treated with U0126 for 1 hour,
and then the embryos were fixed and stained with either anti-phospho-ERK
antibody (four cell) or anti-phospho-ELK1 antibody (two cell). Fluorescence
was viewed with a confocal microscope.
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