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Fig. 6. Helt induces Lim1⁺ neurons and suppresses Brn3a⁺ neurogenesis. (A) Effects of the Helt-null mutation on Lim1 and Brn3a expression. In the Helt^-/- mutant at E11.5, Lim1 expression is lost in the m2 to m4 domains, whereas expression in the m5 and m6 domains is unaffected. At E13.5, Lim1 expression in the dorsal m1 domain is completely lost. By contrast, Brn3a is ectopically expressed in the m2 to m4 domains of the mutants at E11.5. In the m1 domain, virtually all the emerging neurons are positive for Brn3a at E13.5 and coexpression of GFP and Brn3a is observed. (B) Exogenous Helt suppresses Brn3a⁺ neurogenesis and induces Lim1⁺ neurons. In the area efficiently expressing exogenous Helt, many Lim1⁺ neurons are generated, but fewer Brn3a⁺ neurons emerge compared with wild-type control mouse embryos. Exogenous Helt-induced Lim1⁺ neurons express Gad65.

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