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Figure 8


Fig. 8. Monitoring epidermal Notch targets for their expression in hair follicles. (A) Quantitative reverse transcriptase (qRT)-PCR for Notch1, Nrarp, Hes1 and Heyl. Notice that Hes1 expression was sustained in embryo-deleted Notch1 (em-N1) hair follicles. Error bars represent s.e.m. (B) mRNA (Notch1, Nrarp and Cdkn1a) and protein (Hes1) expression in skin. Hes1 (red) expression is found in differentiating hair matrix and epidermal suprabasal cells. AE13 (green) is a marker for follicular cortex and cuticle. Nuclei were stained with DAPI. anN1, anagen-deleted Notch1; WT, wild type. Scale bars: 40 µm.





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