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Figure 2


Fig. 2. SoxN is necessary and sufficient to activate svb expression/direct denticle formation. (A,B) svb RNA expression in stage-14 wild-type (wt; A) and SoxNGA1192 (B) embryos. (C) Ectopic denticle formation in an enGal4-UAS-SoxN larva. The arrow indicates ectopic denticle formation in the anterior row of the En-expressing stripe. Rows are labeled 1-6. (D,E) Misexpression of SoxN with the armVP16Gal4 driver results in ectopic denticle formation (indicated by brackets; D) in those areas where ectopic expression of svb occurs (E); note that svb is expressed in broad stripes but is not ubiquitous. (F) Anti-GFP staining (green) of stage-12 armVP16Gal4-UAS-SoxN-YFP embryos; note that armVP16Gal4 drives SoxN expression in broad stripes but is not ubiquitous. The red staining indicates the ventral midline. (G-I) Misexpression of SoxN with the wgGal4 (wgGal4-UAS-SoxN-YFP) driver results in ectopic denticle formation. (G) Ectopic denticle formation in wgGal4-UAS-SoxN-YFP larvae. (H) Cuticle and anti-YFP staining of stage-17 wgGal4-UAS-SoxN-YFP embryos; note that SoxN directs ectopic denticle formation in a cell-autonomous manner. (I) Cuticle and anti-HA staining of stage-17 wgGal4-UAS-SoxN-UAS-wg embryos; note that concomitant misexpression of SoxN and wg results in ectopic denticle formation in a cell-autonomous manner.





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