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Fig. 5. Dichaete is co-expressed and co-regulated with SoxN and is required for
denticle formation. (A) Double-staining of wild-type (wt) stage-15
embryos with anti-SoxN (green) and anti-Dichaete (anti-D; red); notice the
complete overlap between SoxN and Dichaete expression (yellow). (B)
Staining of stage-15 wgcx4 mutant embryos with anti-D;
notice that Dichaete is ectopically expressed. (C) Staining of stage-15
armVP16Gal4-UAS-EgfrDN embryos with
anti-D; notice the reduction in Dichaete expression. (D) Wild-type
cuticle. (E) SoxNU6-35 mutant cuticle. (F)
SoxNU6-35;D/+ mutant cuticle.