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Fig. 1. Distribution of GABA and Nkx2.1 immunoreactivity in the developing
turtle telencephalon. (A-C) Detection of GABA in coronal sections
of embryonic turtle brains from the level close to the optic chiasm region. At
stage 14 (A), cells above the VZ of the pallium are GABA immunonegative. By
contrast, the VZ of the subpallium shows an intense neuropil labeling and the
mantle contains darkly stained cells. At stage 16 (B), tangentially oriented
GABA-immunoreactive cells are present throughout the entire pallium with the
exception of the dorsal-most sector. (Inset) Enlarged view showing neurites
ending with large growth cones (white arrows). By this stage, the density of
GABA-positive cells is increased in the VZ of the dorsal and medial ridges
(the LGE and MGE, respectively) of the subpallium, which are separated by a
slight depression (dashed line). At stage 19 (C), GABA-immunopositive cells
distribute more medially in the dorsal pallium. A clear transition in GABA
staining occurs at the pallial-subpallial boundary dorsal to the sulcus
between the enlarged DVR and LGE (arrowhead). GABA-positive cells still
concentrate in the VZ of the LGE. (D-F) Detection of Nkx2.1 in coronal
sections of embryonic turtle brains using fluorescent secondary antibodies.
The depression or groove (dashed line) separating the LGE and MGE correlates
well with the dorsal limit of Nkx2.1 expression in the subpallial VZ (white
arrow). Nkx2.1-positive cells distribute in the mantle of the MGE from stage
14 (D), in the mantle of the LGE at stage 17 (E), and in the striatum, DVR and
LC at stage 20 (F). (Inset) Enlarged view showing sparse immunopositive cells
in the DC. DC, dorsal cortex; DVR, dorsal ventricular ridge; LC, lateral
cortex; LGE, lateral ganglionic eminence; m, mantle; MGE, medial ganglionic
eminence; P, pallium; SP, subpallium; Str, striatum; VZ, ventricular zone.
Scale bars: 500 µm.
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