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Figure 5


Fig. 5. Homotopic grafts. (A-G) Trunk and tail of embryos showing incorporation of GFP-labelled cells in homotopic grafts, as indicated. Transverse confocal sections through representative embryos are shown in panels labelled with a lowercase letter. Sections Aa...Ga, mid trunk; Ab...Gb, rostral tail bud (chordoneural hinge, CNH); Ac...Gc, the bud caudal to the notochord end. (Ca, arrowhead) Labelling in intermediate mesoderm. (Da, inset) Transverse confocal section through a different embryo showing endothelial contribution of labelled cells. (Fa) Top section, rostral-most unilateral contribution in the somites in lateral 1-3 grafts (arrowhead in F and H); bottom section, caudal unilateral neural tube and bilateral somite contribution (asterisk in F and H). White arrows, notochord; white boxes in Bb and Fb, CNH. (H) Diagram showing contribution of the lateral border and lateral 1-3 regions to the somites and neural tube. The rostral-most somite labelled is represented as average±standard deviation. s, somite; nt, neural tube; TB, tail bud; TBM, tail bud mesoderm.





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