DEV02880 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Bmp4-induced non-neural cells express E-cadherin. Immunostaining for E-cadherin and keratin 14, counterstained with DAPI (A) and phase-contrast micrograph (B) of wild-type ES cells treated for 5 days with 10 ng/ml Bmp4 in N2B27 medium without LIF. Scale bar: 50 μm.

• Supplemental Figure S2 -

  Fig. S2. Erk1/2 distribution in undifferentiated ES cells. (A,B) Immunostaining of total Erk1/2 (A) or active phospho-Erk1/2 (B) and Oct4 in self-renewing ES cell colonies. (C) Sequential immunostaining of phospho-Erk1/2 and phospho-histone H3 (marker of cells in mitosis). (D) Confocal microscopy of ES cells immunostained for phospho-Erk1/2 and the nucleolar marker fibrillarin, and counterstained with DAPI. Scale bars: 50 μm in A-C; 10 μm in D.