DEV006296 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Montages of sections through lhw and WT roots. Original confocal sections used in the stacked images provided in the text. In each pair, propidium iodide is on the left and J0121::GFP is on the right. (A,B) WT, from Fig. 1B; (C,D) lhw, from Fig. 1C; (E,F) lhw treated with 2,4 D, from Fig. 3C.

• Supplemental Figure S2 -

  Fig. S2. Embryo and lateral root development in lhw and wild type. (A-H) DIC images of cleared embryos. (A,B) Early globular embryo of WT (A) and lhw-1 (B); (C,D) late globular WT (C) and lhw-1 (D); (E,F) late heart of WT (E) and lhw-1 (F). (G,H) Higher-magnification image of the basal pole of an embryo showing divisions in WT (white arrows in G) but no cell divisions in lhw-1 (H). (I,J) Confocal images of torpedo-stage WT (I) and lhw-1 (J) embryos. The width of vascular cylinder is indicated by white (WT) and yellow (lhw-1) bars. Both bars supplied for comparison in J. (K-P) WT and lhw-1 lateral root primordial expressing CYCB1;1::GUS (blue). (K-N) Comparison of longitudinal extent of lateral root initiation stages as in Malamy and Benfey (Malamy and Benfey, 1997), stage 1 (K-L) and stage 2 (M-N). (O,P) Head-on view of stage-1-2 lateral root showing three contiguous files (black asterisks) undergoing divisions in both WT (O) and lhw-1 (P). Each staged WT and lhw image pair is at same magnification.

• Supplemental Figure S3 -

  Fig. S3. Comparison of WT and lhw root growth inhibition. Graph of root length at 7 dpg in response to germination and growth on increasing 2,4D concentrations. WT (open diamonds) and lhw-1 (filled squares).

• Supplemental Figure S4 (Word Doc) -

  Fig. S3. Comparison of WT and lhw root growth inhibition. Graph of root length at 7 dpg in response to germination and growth on increasing 2,4D concentrations. WT (open diamonds) and lhw-1 (filled squares).