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Fig. 3. Local tissue deformation in wild-type, eve and string
embryos during the fast phase of germband extension.(A,B)
Individual nuclei (and their progeny) at the posterior of a wild-type embryo
were tracked during elongation. No intercalation was detected during this
period despite clear tissue elongation. (C-H) In order to assess tissue
deformation, a group of 25 nuclei in wild type (C,D), eve mutants
(E,F) and string mutants (G,H) was outlined before (7 minutes after
the posterior tip came into view; C,E,G) and after (13 minutes after the
posterior tip came into view; D,F,H) a cluster of cell divisions. Deformation
of the outline gives a visual indication of tissue deformation. To obtain a
more quantitative assessment, a measure of the aspect ratio (AR) of the
outlines was devised (as described in I). Overall, the data suggest
that tissue elongation in this region of the germband is reduced in
eve mutants and is nearly absent in string-deficient
embryos.
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