|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Core planar polarity gene function is required in the border cells.
Anterior is to the left and border cells are migrating towards the right, in
this and subsequent figures. Statistical significances are indicated on charts
as ***P<0.001 and **P<0.01; all
P values and numbers of clusters examined are shown in Table S1 in
the supplementary material for UAS/GAL4 experiments and Table S2 in the
supplementary material for mosaic cluster analysis. (A) Schematic of
border cell migration and outer follicle cell rearrangement. Anterior polar
follicle cells (red) recruit adjacent outer follicle cells (light green) to
form the border cells (dark green). The border cell cluster delaminates from
the follicular epithelium and begins to migrate posteriorly at the beginning
of stage 9, normally completing its journey by the end of this stage.
Concomitantly, the outer follicle cells rearrange so that they no longer cover
the nurse cells. In wild-type chambers, the border cell cluster migrates at
such a rate that it approximately keeps up with the posterior movement of the
outer follicle cells. (B,C) Charts showing the extent of border
cell migration relative to outer follicle cell rearrangement for clusters in
which either fz, dsh or stbm transcripts have been either
knocked-down by UAS-RNAi constructs at 29°C (B) or overexpressed
using UAS constructs at 25°C (C) under the control of the border
cell-specific slbo-GAL4 driver
(Rørth et al., 1998).
Coloured bars indicate the proportion of clusters found ahead of the outer
follicle cells, in approximately the same position (`normal') or lagging
behind, for either sibling controls or experimental conditions. `Ahead' or
`behind' are defined as being more than the diameter of a nurse cell nucleus
away from the trailing edge of the rearranging outer follicle cells. Either
knockdown of fz, dsh or stbm or overexpression of
fz or stbm causes a significant increase in the number of
clusters `behind' and an accompanying decrease in the number of clusters
showing a `normal' rate of migration. (D) Chart showing the proportions
of genetically mosaic clusters recovered for the strong alleles
fz15 and stbm6 with both polar
follicle cells retaining gene function, but with either wild-type border cells
leading (pink bars) or mutant border cells leading (blue bars). In both
genotypes, there is a statistically significant (P=0.003)
preponderance for wild-type border cells to be found at the leading edge of
the migrating cluster. Mutant cells in the cartoons are represented by grey
shading, with leading cells to the right and lagging cells to the left.
|
