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Fig. 2. Border cell clusters lacking core planar polarity gene function show normal expression of slow border cells, DE-Cadherin and Stat92E. (A-D) slow border cells expression as revealed by the slbo-lacZ reporter (Montell et al., 1992); ß-gal immunolabelling (red) and DE-Cadherin (DE-Cad) expression (green) in migrating border cell clusters from wild-type (A), fz²¹/fz¹⁵ (B), stbm⁶ (C) and dsh¹ (D) individuals. High levels of nuclear-localised lacZ gene product in border cells is indicated by arrowheads. We observed that, in wild-type clusters, ß-gal levels were lower at early stage 9 than at the end of stage 9, whereas, in mutant clusters, ß-gal levels were generally higher throughout migration. We assume that ß-gal accumulates progressively within the border cells after the onset of gene expression, and that the delayed migration seen in the mutant backgrounds results in higher accumulation at equivalent stages of migration. (E-H') DE-Cad (green/white) and actin (red) distribution in migrating border cell clusters from wild-type (E,E'), fz²¹ (F,F'), stbm⁶ (G,G') and dsh¹ (H,H') individuals. Border cells are marked by red dots and polar follicle cells by white asterisks. (I-K) Stat92E (green) and Armadillo (Arm, red) distribution in migrating border cell clusters from wild-type (I), fz²¹ (J) and stbm⁶ (K) individuals. High levels of nuclear-localised Stat92E in border cells is indicated by arrowheads.

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