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Figure 5


Fig. 5. Fz and Stbm proteins are localised within the border cell cluster. Illustrations (top) show border cells (white), polar follicle cells (grey) and Fz (red); the direction of migration is towards the right (grey arrows). (A-D) Border cell clusters stained for Fz (red) and actin (green). Fz is localised in the adherens junctions of the polar follicle cells (A) and apical regions of the border cells (B) prior to migration. During migration, Fz localisation is retained in the junctional region that the polar follicle cells share with the border cells (C) and is within the migratory regions of the border cells (D). This pattern of localisation is lost in egg chambers mutant for fz, consistent with the immunolabelling being specific (data not shown). (E,G) Egg chambers stained for Armadillo or actin (red) and Stbm (green). Stbm is localised to the polar follicle cell adherens junctions (arrow). This pattern of localisation is lost in egg chambers mutant for stbm (data not shown). (F,H) Egg chambers stained for Armadillo or actin (red) and Dsh-GFP (green). Dsh-GFP is seen in a punctate pattern in polar follicle cell and border cell cytoplasm, and also partially overlaps the adherens junction region (arrow).





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