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Fig. 1. Growth phenotype of wild-type and er-family mutant
Arabidopsis. (A-H) Wild-type and er-family mutant
inflorescence stems. (A) Wild type (WT); (B) er-105; (C) er-105
erl1-2; (D) er-105 erl1-2 erl2-1; (E) er-105 erl1-2
erl2-1/+; (F) er-105 erl1-2/+ erl2-1; (G) er-105 erl1-4
erl2-1/+; and (H) er-105 erl1-1 erl2-1/+. Wild type and all
combination mutants (A-C,E-F) except er-105 erl1-2 erl2-1 triple
mutant (D) maintain proper floral organ patterning and elongation. Among the
combinations, er-105 erl1-2 erl2-1/+ (E), er-105 erl1-4
erl2-1/+ (H) and the triple mutant (D), are female sterile. Scale bar: 1
cm. (I) Schematic of the ERL1 gene with location of T-DNA
insertions for erl1-1 and erl2-1, and the point mutation for
erl1-4. Exons are indicated as vertical bars, introns as lines. The
T-DNA right border (RB) is boxed. (J) RT-PCR analysis of four separate
erl1-1 plants with reduced levels of ERL1 transcript
compared with wild type. ACTIN 2 serves as a positive control.