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Fig. 4. ERL2 is haploinsufficient to maintain integument growth.
(A,B) Transverse (horizontal) sections of Arabidopsis
ovules at anthesis. (A) er-105 erl1-2/+ erl2-1 has a fully encased
and developed embryo sac. (B) er-105 erl1-2 erl2-1/+ ovule has
shorter integuments, protruding nucellus with no observable embryo sac.
(C) Mean cell area (±s.e.m.) of the outer integument cells of
wild type and er-105 erl1-2 erl2-1/+ (n=25, Student's
t-test, P=0.331). (D) Cell number (±s.e.m.) in
epidermis of ovules (stage 1-II) and the outer layer of the outer integument
(stage 2-III, 3-III and 4-I) of wild-type and er-family mutant ovules
at the indicated stages (n=25). Asterisks indicate a significant
difference from the respective wild type within each stage using Student's
t-test (P=0.01). At stage 3-III, a decrease in the number of
cells in the outer integument is detected between wild type and
er-family mutants. By stage 4-II, the number of cells in the outer
integument is dramatically reduced compared with wild type. (E)
Real-time RT-PCR analysis of selected cyclins in stage 12 carpels (left) and
stage 3-II ovules (right). Loss of ER-family function results in a disruption
of cyclin gene expression. Scale bars: 20 µm.
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