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Figure 1


Fig. 1. S. mediterranea innexin gene expression analysis. (A) Representative S. mediterranea innexin expression patterns in wild-type animals were organized into four groups: (1) nervous: central nervous system (CNS) and marginal cells (smedinx-3 and smedinx-4), CNS and pharynx opening (smedinx-5); (2) mesenchymal tissue (smedinx-1, smedinx-2, smedinx-6, smedinx-7, smedinx-8, smedinx-11 and smedinx-12), which is widely distributed and might include more than one cell type (e.g. pharynx, CNS and diverse sub-epithelial cells); (3) excretory system: flame cells (smedinx-10); and (4) digestive: gastro-vascular system (smedinx-9). In all panels, anterior is up and, except for the CNS group, which is ventral, dorsal views are shown. For each probe, n≥18 animals (results were consistent in each case). (B) Anterior transverse section (8 µm) at the level of the photoreceptors/brain showing specific expression of smedinx-11 in individual cells. Red arrowheads indicate representative expressing cells. CG, cephalic ganglia; PR, photoreceptor. Scale bars: 0.2 mm in A; 20 µm in B.





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