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Fig. 3. smedinx-11(RNAi) phenotype. (A) Early phenotype:
lack of regeneration. Regenerating fragments at 7 days post-amputation;
control animal (left) formed AP blastemas (lightly pigmented new tissue
indicated with white arrows), whereas smedinx-11(RNAi) fragments
failed to create new tissue (30/30) even at 3 weeks post-amputation (red
dotted lines represent plane of amputation, animals were amputated 8 days
after first injection). (B) Late phenotype. Intact animals; control
(left) and smedinx-11(RNAi) representative images show progression of
the phenotype. Top scale indicates days after first injection of
smedinx-11 dsRNA. Initial signs of the phenotype are visible 2 weeks
after first injection and were characterized by a contraction of the
pre-pharyngeal region (white arrows at 14 days). At 21 days, the lateral and
posterior edges of the worms curled under ventrally (white arrows) (50/50).
Unlike irradiated, smedwi-2(RNAi) or smedbruli(RNAi) worms,
animals with the smedinx-11(RNAi) phenotype do not develop head
regression even 1 month after first exposure to smedinx-11 dsRNA
(13/15 worms, yellow arrow at the front of the animal at 31 days).
Simultaneous smedinx-11 + smedinx-2 double-knockdown phenotype in
both intact and regenerating fragments did not show differences when compared
to smedinx-11(RNAi) alone (data not shown). All animals died 35-40
days after first injection. In all cases, anterior end is up. Scale bars: 0.2
mm.
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