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Fig. 3. Morphant embryos show defective fast-myoblast fusion. (A)
Embryo injected with dock5 splice 1 morpholino, showing examples in
neighbouring somites of GFP-positive mononucleate cells that (left) span the
somite and (right) have failed to elongate. (B,C) Embryo
injected with dock5 splice 2 morpholino, showing an example of
defects in somite shape. B is more medial and shows that the GFP-positive
cells are medial to the superficial slow fibres shown in C, the slow fibres in
B being the medially located muscle pioneers. (D) Embryo injected with
crkl ATG morpholino, showing an example with many mononucleate
GFP-labelled cells that have failed to elongate and still extend thin
processes. (E,F) Embryo injected with dock5 splice 1
and crkl ATG morpholinos combined. Slow fibres are unaffected (F),
although there are many mononucleate GFP-positive fast fibres (E). Circles in
E indicate the positions of nuclei. All morpholinos were injected in solution
containing mylz2:GFP plasmid and were fixed at 26-28 hpf. Green, GFP
of mylz2:GFP plasmid; red, F59-antibody slow-fibre stain. Scale bars:
25 µm.
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