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Fig. 2. Time-lapse analysis and branching. (A) Individual movie
frames (see Movie 1 in the supplementary material) showing wild-type CaP/VaP
migration. Notice the growth cone from an interneuron extending along the
ventral aspect of the spinal cord (arrowhead). (B) Individual movie
frames (see Movie 2 in the supplementary material) showing the ectopic exit of
a sidetracked MiP/RoP axon (asterisk). The soma is located between
two CaP/VaP pairs. Mutant growth cones pioneer an ectopic exit zone into the
periphery, where they often branch (yellow arrow) and sometimes join
endogenous motor axons (white arrow). Notice the growth cone from an
interneuron extending along the ventral aspect of the spinal cord (arrowhead).
sidetracked mutants display excessive branching. (C,D)
24 hpf embryos injected with Hb9:GFP plasmid, and stained with
anti-GFP (green) and SV2 (red) to visualize CaP, MiP and RoP trajectories,
respectively. (C) Wild-type (wt) embryo. (D) sidetracked embryo.
Notice the small branches along the axonal shaft. (E,F) 48 hpf
wild-type and sidetracked embryos stained with znp1 and SV2. Notice
the persisting ectopic exit points (arrows) and the long branches (arrowheads)
present in the mutants. CaP, caudal primary; MiP, middle primary; RoP, rostral
primary.
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