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Figure 1


Fig. 1. FoxF expression in anterior B7.5 cells requires Ets1/2 activity. (A-C) In situ hybridization on neurula (A,B) and tailbud (C) embryos. Arrowheads (A,B) indicate the anterior B7.5 cells, which migrate and form the trunk ventral cells (TVCs; white arrowheads in C). Notice the FoxF expression in trunk epidermal cells. (D-G'') The B7.5 lineage cells were visualized with an anti-ß-galactosidase antibody (red), and FoxF expression was revealed by fluorescent in situ hybridization (green). (D-D'') Embryo electroporated with the Mesp>lacZ control. Within the B7.5 lineage, FoxF is only expressed in the anterior TVCs and not in the posterior tail muscles. (E-E'') Embryo co-electroporated with Mesp>lacZ and Mesp>Ets:VP16. Ets:VP16 induces all four B7.5 lineage cells to migrate and express FoxF. (F-F'') Embryo co-electroporated with Mesp>lacZ and Mesp>Ets:WRPW. Ets:WRPW inhibits both the migration of B7.5 cells and FoxF expression. (G-G'') Embryo co-electroporated with Mesp>lacZ and Mesp>Mesp:VP16. All B7.5 cells remain in the tail; anterior cells fail to express FoxF.





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