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Fig. 3. Timecourse analysis of SNAP25
20-induced oocyte maturation
in Xenopus. (A) Extent of GVBD (black) and membrane
capacitance (blue) in progesterone- (left panel) and SNAP2520- (right
panel) treated cells. The inset (below left) shows no change in capacitance or
maturation in untreated oocytes over a similar timecourse. (B) MAPK and
MPF activation and SNAP25 expression over the maturation timecourse following
SNAP2520 mRNA injection (left panel) and progesterone treatment (right
panel). (C) Oocytes were either left untreated and matured with maximal
levels of progesterone (16 µM), or injected with the light chain of BoNT A
(200 nM) or BSA as the carrier control and matured with sub-threshold levels
of progesterone (100 nM). The maturation timecourses for the three treatments
show that BoNT A injection potentiates the effects of sub-threshold levels of
progesterone on maturation. This experiment was repeated four times on oocytes
from different donor females, and the potentiation effects of BoNT A were
observed in 2/4 experiments. (D) Membrane capacitance (Cm)
measurements at different times, as indicated, after BSA or BoNT A (200 nM)
injection, showing that BoNT A is ineffective at reducing Cm.
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