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Figure 4


Fig. 4. Activation of adenylate cyclase inhibits SNAP25{Delta}20-induced maturation. (A,B) Extent of maturation of Xenopus oocytes treated with progesterone or injected with SNAP25{Delta}20 mRNA in the presence or absence of 100 µM forskolin (A) or 5x10-9 M cholera toxin (B). Timecourses of maturation were carried out and the plateau levels of GVBD at the end of the experiments are reported (above). The activation of MAPK and MPF and the expression level of SNAP25{Delta}20 are also shown (below). Lysates for western blot analysis were prepared from cells at the GVBD50 time point. (C) Percent GVBD of Mos mRNA- (10 ng) injected oocytes in the presence or absence of 100 µM forskolin or 5x10-9 M cholera toxin. The inset (below) shows the time to GVBD50 for Mos-injected cells±forskolin. CT, cholera toxin; Forsk, forskolin.





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