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Fig. 7. Ectopic expression of porcupine in the chick neural tube steepens the
Wnt1/Wnt3a proliferation gradient. mPorcD was transfected into the neural
tube by electroporation. GFP-positive embryos were harvested for analysis 48
hours post-electroporation. Embryos were fixed, cryosectioned and
immunostained with anti-phosphohistone H3 (red), which marks cells in late
G2/M phase. The neural tube was divided into quintiles for quantitation of
proliferation, as illustrated in schematic to left. The red dots represent
mitotic cells in each quintile. The proliferation index for the control and
experimental sides of the neural tube was determined and graphed for each
quintile. A total of 43 sections derived from three different embryos were
analyzed. Error bars indicate standard error.
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