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Figure 1


Fig. 1. Hypoxia blocks culmination in the normal Dictyostelium strain Ax3. (A) Cells were developed for 25 hours on filters in an atmosphere of the indicated percentage of O2 with N2. Similar results were obtained in the presence of 1% CO2 (not shown). (B) Timecourse of effect of O2 shift on culmination visualized qualitatively at 20 hours. (C) Effect of hypoxia on cell proliferation. Ax3, P4H1oe (HW285) or P4H1- (HW288) cells (see later) were diluted to 106 cells/ml (indicated by dashed line) in growth medium (HL-5) and shaken in the indicated percent of O2 in N2. Cells were counted 24 hours later. (D) Effect of overexpressing PKAcat on the hypoxic blockade, quantitated by counting detergent-resistant spores after 36 hours. Spore numbers correlated with morphological culmination (not shown). Results are typical of three independent trials.





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