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Fig. 4. BMP signalling is required in the epiblast for mesoderm specification
and to inhibit neural differentiation. (A,B) At 5.5 dpc,
pSmad1/5/8 expression is slightly decreased in the epiblast of
Bmpr1a-/fx; Sox2Cre+/- mouse embryos
(n=4/7) as compared with controls. (C,D) At 6.5 dpc, in
Bmpr1a-/fx; Sox2Cre+/- embryos pSmad1/5/8
expression is severely reduced in the epiblast (n=4/7) and
(E,F) by 7.5 dpc is almost lost (n=4/4), although some
expression in the visceral endoderm remains at both these stages.
(A'-F') Overlay of pSmad1/5/8 and nuclear staining.
(G,H) At 6.5 dpc, Fgf8 is expressed in the posterior
epiblast of controls but its expression is reduced or lost in the majority of
Bmpr1a-/fx; Sox2Cre+/- embryos
(n=4/6). (I,J) Cripto is expressed in the
posterior epiblast of controls at 6.5 dpc but is lost in a proportion of
Bmpr1a-/fx; Sox2Cre+/- embryos
(n=3/9). (K,L) At 6.5 dpc, Nodal is expressed
in the posterior epiblast of controls, but when Bmpr1a is deleted
from the epiblast Nodal expression is reduced or lost
(n=3/4). (M,N) At 7.25 dpc, Hesx1 is expressed
in the anterior definitive endoderm and anterior neural ectoderm of controls.
A large expansion in the domain of Hesx1 expression is observed in
the epiblast of Bmpr1a-/fx; Sox2Cre+/- embryos
(n=4/6). (O,P) Six3 is a marker of the
anterior neural ectoderm at 7.5 dpc. In Bmpr1a-/fx;
Sox2Cre+/- embryos, Six3 expression is greatly
expanded (n=7/8). (Q,R) At 7.5 dpc, Sox1 is
not expressed in control embryos but can be found throughout the epiblast of
Bmpr1a-/fx; Sox2Cre+/- embryos. All are lateral
views, anterior to the left.
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