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Figure 1


Fig. 1. Sites of electroporation and domains in the endoderm for fate mapping experiments. (A) Regions of the endoderm for testing cell fates by electroporation: anterior-proximal (AP), anterior-distal (AD), distal (Dist), lateral (Lat), posterior-distal (PD), posterior-middle (PM) and posterior-proximal (PP). (B) Regions of the endoderm for scoring the distribution of GFP-expressing cells in early-head-fold-stage embryos. (C-C'') Early-somite-stage embryo. The sub-division of the endoderm is shown as a schematic lateral view (C), an oblique view (C') and a ventral view (C''). In the early-somite-stage embryo, although the domain of embryonic foregut may be approximately delineated by the foregut portal, the precise sub-division of gut segments is not morphologically evident, and a substantial length of the mid- and hind-gut is yet to be formed. Embryonic endoderm of the anterior (A, rostral segment of the foregut), middle (M, posterior segment of the foregut and endoderm at the somitic level) and posterior (P, endoderm associated with the presomitic mesoderm) regions. AYS, anterior yolk sac; LYS, lateral yolk sac; PYS, posterior yolk sac.





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