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Fig. 2. Localization of EGFP and lacZ-expressing cells in the endoderm
following electroporation of expression vectors. Distribution of
EGFP-expressing cells at the mid-streak (MS) stage (3 hours), at the
early-head-fold-stage (24 hours) and in the early-somite-stage embryo (46-48
hours) after electroporation of the endoderm of mid-streak-stage (E7.0)
embryos at seven sites: (A) lateral, (B) anterior-proximal,
(C) anterior-distal, (D) posterior-proximal, (E)
posterior-middle, (F) posterior-distal and (G) distal. Examples
of the pattern of distribution of EGFP-fluorescent cells after electroporation
and in vitro culture are shown as a set of three (at 3 hours, 24 hours and
46-48 hours) for each site. All figures are lateral views with anterior to the
left, except for anterior views of anterior-proximal (24 hours and 46-48
hours) and anterior-distal (46-48 hours), and posterior views of
posterior-proximal (24 hours and 46-48 hours) and posterior-middle (46-48
hours) sites. (H-L) Localization of the electroporated
lacZ-expressing cells in (H,J) whole-mount and (I,K,L) histological
sections of the embryo. (H,I) Endoderm of an E7.0 MS-stage embryo after 3
hours of in vitro culture. (J-L) endoderm of the dorsal foregut (K) and the
middle region (L) of an early-somite-stage embryo (J) after 24 hours of in
vitro culture. The faint staining in I at the basal region of the epilast is
probably due to the spillage of the product of the X-gal-staining reaction, as
opposed to genuine staining, which would be found in the whole cell.
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