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Fig. 5. Altered distribution of dividing cells, T-cell number and apoptosis in
K14-Gata3-/- skin. (A-F) BrdU labeling of
dividing cells of wt (left) and K14-Gata3-/- (right) skin.
In wt animals, BrdU labeling (brown-stained nuclei) is mostly present in the
bulb of the HFs (D). The cells of the basal layer of the
K14-Gata3-/- epidermis are dividing at a much higher rate
(compare A with B). There is no obvious difference in the number of
BrdU-labelled HF cells, but in their distribution (compare regions a and b in
D-F). (G-I) TUNEL assay shows altered apoptosis in
K14-Gata3-/- skin as compared with control skin at P11.
Arrows indicate positive cells (J-L): K14-Gata3-/-
skin contains less CD3 positive T-cells (red dots, arrows) than wt skin. White
asterisks mark background staining of an artifact of the knots of highly
keratinized material (G,H) and sebaceous glands (J,K). Data are given as
mean±s.e.m.; *, P
0.05; Student's
t-test. Scale bar in A: 150 µm for A,B,D,E,J,K; 100 µm for
G,H.
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