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Fig. 6. The level and localization of Robo are not altered in
ß-spectrin null embryos. (A,D) Embryos stained
with anti-HRP. (B,E) Embryos stained with anti-Robo.
(C,F) Merged image for each genotype. ß-spectrin
mutant embryos (D-F) do not show reduction or mislocalization of Robo,
although the distance between opposing sides of the midline is reduced when
compared with wild-type embryos (A-C). Embryos were processed in parallel and
images were taken at the same confocal settings.