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Fig. 6. Heat-shock induction of NICD. (A-D) Expression of
atoh1b and ngn1 at 11 hpf (A,B) and 12 hpf (C,D) in control
embryos (A,C) or NICD-positive embryos (B,D) heat shocked at 9 hpf. Loss of
ngn1 expression, which is non-overlapping with atoh1b,
confirms effective NICD-induction. (E-H) Expression of atoh1a
at 19 hpf (E,F) and 20 hpf (G,H) in atoh1b morphants without NICD
(E,G) or with NICD (F,H) heat shocked at 18 hpf. Otic vesicles are outlined.
Arrowheads mark otic expression domains. All are lateral views with anterior
to the left. Scale bar: 15 µm. tg, trigeminal ganglion.
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