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Fig. 4. Expression of Gli1 and gremlin is altered in the forelimbs of
prx1cre;Ift88fl/n conditional mutants. Whole-mount in
situ hybridization analysis of Gli1 (A-D) and gremlin
(E-H) expression in wild-type (A,C,E,G) and
prx1cre;Ift88fl/n (B,D,F,H) forelimbs at E10.5 (A,B,E,F)
and E11.5 (C,D,G,H). At E10.5, Gli1 expression is similar in
conditional mutants (B) and wild-type controls (A). At E11.5, the expression
of Gli1 is retained in wild-type controls (C) but is almost
completely absent in conditional mutants (D), except small patches of cells
expressing Gli1 can occasionally be seen in
prx1cre;Ift88fl/n mutants (arrowhead). Gremlin expression
is restricted to the posterior mesenchyme at E10.5 in wild-type forelimbs (E)
but is expanded into the anterior mesenchyme in
prx1cre;Ift88fl/n conditional mutants (arrowhead in F).
Expression of gremlin is minimal in wild-type forelimbs at E11.5 (G). By
contrast, in prx1cre conditional mutant forelimbs (H), ectopic
anterior expression of gremlin is maintained (arrowhead in H). Anterior is up
in all panels.
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