|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Drosophila transgenesis. white+
transgene DNA (red) is injected into generation zero Drosophila
embryos (G0) of less than 1 hour old, which have been obtained from a parental
(P) generation. The early developmental stages of Drosophila embryos
are characterized by rapid nuclear divisions that occur without accompanying
cell divisions, creating a syncytium. Prior to cellularization, pole cells
(black) bud off at the posterior end. For germ line transmission to occur, the
transgenic DNA must be taken up into the pole cells that are fated to become
germ cells. Transgenic DNA integrated into a pole cell (red pole cell) can be
transmitted from one generation (G0) to the next (G1 progeny). The resulting
integration events are identified using an appropriate marker, such as as
white+. When used in a mutant white-
strain, this transgene marks transgenic flies by giving them a darker eye
color (see Table 2 and
Box 3 for more information on
the markers used in fly transgenesis).
|
