|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Ubx and Mad/Med binding sites collaborate to repress sal
expression in the haltere. Schematics above each panel highlight the
relevant changes to CREs. (A,B) Haltere imaginal discs from
transgenic Drosophila carrying sal1.1 reporter constructs
with mutations either in Ubx binding sites 5 and 6 or in the M1 site. The
level and pattern of derepression is identical whether Ubx sites 5 and 6 or
the M1 site are mutated. (C) Mutations in Ubx binding sites 5 and 6 in
a subelement of the sal1.1 CRE, sal328, derepress the
reporter gene lacZ in haltere discs. (D) The addition of one
copy of Ubx binding site 5 to both the 5' and 3' ends of
sal328koU5&6 (sal328koU5&6+2-U5) is insufficient to
repress reporter gene expression in the haltere. (E) The vgB
CRE is expressed along the dorsal-ventral compartment boundary in both the
wing (W) and haltere (H) imaginal discs. (F) The addition of two
cassettes, each containing four copies of Ubx binding site 5, to the 5'
and 3' ends of the vgB is not sufficient to impart
Ubx-dependent repression in the haltere imaginal disc.
|
