DEV005280 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Close proximity between the PE and liver bud. (A) Ventral view of a stage 16 chick embryo. To expose the PE, the pericardiac membrane was opened and pinned to the bottom of the dish. (B) High magnification view of the embryo shown in A. The PE can be recognized as villous protrusions located dorsal and posterior to the heart tube (h). (C) A sagittal section of a stage 16 embryo stained with Hematoxilin and Eosin, showing the close proximity between the PE and underlying liver bud. Anterior, top; posterior, bottom; dorsal, right; ventral, left. The liver bud (LiB) can be identified as an outgrowing bud in the ventral floor of the foregut, which underlies the developing PE. (D) High magnification of the boxed area in C. fg, foregut; mg, midgut. Scale bar: 100 ?m.

• Supplemental Figure S2 -

  Fig. S2. Activity of candidate paracrine factors in regulating PE marker genes. Four cLEF was cultured in serum-free M199 in the presence of candidate factors and effects on gene expression monitored by RT-PCR, using cWt-1, cTbx-18, cCfc, cPax-2 and cGAPDH primers. The amount of PCR product for each gene was quantified on an agarose gel and normalized to cGAPDH. Bar indicates normalized band intensity relative to that obtained from the BSA control.