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Figure 5


Fig. 5. Expression of MSI1 before and shortly after fertilization can rescue the female gametophytic msi1 mutant phenotype. (A) DD46::GUS expression can be detected in the female gametophyte (left). Residual expression of DD46::GUS in seeds at 2 DAP (right). (B) Expression of DD46 on microarrays. DD46 is expressed before fertilization (stage I) and is reduced to baseline (dashed line) levels after fertilization (stage II) and during seed development (stage III) [data from Hennig et al. (Hennig et al., 2005)]. (C) msi1; DD46::MSI1 plants pollinated with wild-type pollen do not form aborting seeds. (D) Quantification of seed abortion observed after crosses of wild type (wt)xwt (n=269), msi/MSI1 xwt (n=487), msi1/MSI1; DD46::MSI1 #1 xwt (n=369), msi1/MSI1; DD46::MSI1 #2 xwt (n=475). (E) Cleared seeds of msi1/MSI1; DD46::MSI1 pollinated with wild-type pollen. Seeds of this cross (right) are indistinguishable from wild-type seeds (left). (F) msi1/MSI1; DD46::MSI1 plants do not form endosperm without fertilization (right). Autonomous endosperm development in msi1 mutants at a similar time point (left). Scale bars: 50 µm in A,E,F; 200 µm in C.





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