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Fig. 1. Ttk tracheal expression and requirements. (A) Late-stage ttk mutant embryo showing an absence of branch fusion in the dorsal trunk (DT) (arrows). (B,C) Ttk69 protein accumulation in wild-type embryos at stage 11 (B) and 14 (C). Ttk (red) is expressed in all tracheal cells (green). (D-F) Embryos at late embryogenesis. Notice the rudimentary aspect of the tracheae in ttk mutants (E,F) as compared with wild type (WT; D). (G) Details of two tracheal metameres, showing the absence or reduction (arrows) of visceral branches (VBs) in a ttk mutant. (H) Schematic representation of wild-type bnl expression (dark grey) at stage 13. (I,J) Expression of bnl at stage 13. Notice the reduction in size and number of bnl spots in the ttk mutant (J). (K) A stage 12 embryo showing co-expression of bnl mRNA and Ttk69 protein (arrows). (L,M) Details of stage 15 embryos labelled to highlight DSRF-positive terminal cells (arrows). DSRF is expressed in several presumptive terminal cells in ttk mutants (arrows in L). All panels, except H, I and J, show projections of confocal sections of laterally viewed embryos. In this and all figures, dorsal is up and anterior to the left.

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