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Fig. 3. Ttk is required during tracheal cell intercalation. (A-D') Projections of confocal sections showing details of stage 13 (A,C) and 16 (B,D) embryos focused at the dorsal trunk (DT) and dorsal branches (DBs). No apparent differences are observed at the early stages (compare A with C). Accumulation of DE-cad (labelled with DCAD2 and shown in greyscale in B' and D') shows the presence of autocellular adherens junctions (AJs; arrow in B') in wild type (WT). (D') ttk embryos exhibit mainly intercellular AJs (arrowhead) and only occasional short stretches of autocellular ones (arrows). (E-F') Projections of confocal sections showing lateral trunk (LT) details of stage 16 embryos. Notice the presence of autocellular AJs in wild type (E'), and the elongated shape of the intercalated cells (E, cells have been coloured to facilitate observation of cell rearrangements and shape). Conversely, ttk mutants show mainly intercellular AJs (F') and cuboidal cells positioned side-by-side (F). (G) Projection of confocal sections showing a late-stage ttk mutant. The formation of autocellular AJs (arrows) is restored by tracheal expression of ttk. (H,I) Lateral views of ttk embryos at stage 13-14 showing normal accumulation of Sal and Kni proteins (arrows). (J,K) Projections of confocal sections showing details of stage 16 wild-type and ttk mutant embryos labelled to show Pio accumulation. (L-N) Expression pattern of pyd at the placode stage (arrows). Notice the absence of expression in ttk mutants (I) and increased levels in several structures (arrows in J) when ttk is generally overexpressed.

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