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Fig. 6. Ttk controls tracheal tube size. (A,B) Portions of
dorsal trunk (DT) in longitudinal views (upper panels) or in cross-sections
(lower panels) of wild type (WT; A) and ttk mutants (B). Thickness of
the tubes is measured. (C,D) Stage 16 embryos showing
accumulation of CBP in the DT. ttk mutants show an abnormal
intraluminal chitin filament (D). (E-G) Expression pattern of
mmy in stage 14 embryos. Notice the increased levels of expression in
ttk mutants (F) and the absence of expression when ttk is
generally overexpressed (G). (H,K) Stage 16 embryos. Verm is
abnormally accumulated in ttk mutants (K).
(I,J,L,M) Stage 16 embryos. Accumulation of
septate junction (SJ) markers (FasIII and Cora; green) in ttk mutants
(L,M) is only slightly affected (L) or is comparable to wild type (M).
(N,O) Stage 16 embryos injected with a 10 kDa rhodamine-labelled
dextran. ttk embryos (O) are permeable to the dye, which fills the
tracheal lumen (arrows), whereas the wild-type trachea is impermeable (N). All
panels except E-G,N and O show projections of confocal sections of laterally
viewed embryos.
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